Use of Whole Genome Amplified DNA for SNP Array Analysis

The amount of DNA available from tissue samples, especially samples from tumor biopsies, is usually limited. Fortunately, whole-genome amplification has been developed to generate micrograms of DNA for further analysis (52,53). We found similar genotype calls and copy number changes in whole-genome amplified DNA with that using unamplified DNA, as determined by a 10 K SNP array analysis (30).

Figure 3 shows a comparison between DNA extracted directly from tumor tissue without microdissection and whole-genome amplified DNA from microdissected tumor cells. LOH was detected in microdissected samples only. Thus, an increase in the sensitivity to copy number aberrations can be achieved using whole genome-amplified DNA from microdissected samples for SNP array analysis.

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