Ic

Assay of total catenated SV40 dimers by pulsed-field agarose gel electrophoresis of DNase I and Bgl I digested SV40 DNA replication intermediates. The left lane shows the pattern ofSV40 intermediates separated by pulsed-field agarose gel electrophoresis. The A-, B-, and C-family catenated dimers are all found in the region between the form II band and the late Cairns structure (LC). Intermediate Cairns structures (IC) are distributed as a continuous smear from the form I band to the...

Editors

Associate Professor of Radiology The Ohio State University Columbus, Ohio, U.S.A. Chapters 1-6, 8 John M. Cassady, Ph.D. Professor and Dean, College of Pharmacy The Ohio State University Columbus, Ohio, U.S.A. Chapter 6 Christopher A. Ferrer, B.S. Graduate Research Associate, Department of Medical Microbiology and Immunology The Ohio State University Columbus, Ohio, U.S.A. Chapter 6 Linda H. Malkas, Ph.D. Associate Professor, Department of Pharmacology University of Maryland School of Medicine...

Chapter

DNA replication can be disrupted by DNA damage as well as inhibition of DNA replication enzymes. DNA damaging agents make up an important class of anticancer drugs that includes DNA nicking agents such as bleomycin, DNA alkylating agents and DNA crosslinking agents. Ionizing radiation, one of the most useful anticancer treatments, targets DNA. DNA damaging agents are also mutagenic and carcinogenic. A great deal is known about the DNA lesions caused by different DNA damaging agents, and the...

Arac

A The HeLa cell MRC was prepared as described in ref. 75. MRC-mediated in vitro DNA replication assays were performed as described75 in the absence or the presence of a range of concentrations of ara-CTP, camptothecin (CPT), and etoposide (VP-16). Dimethylsulfonyl oxide (DMSO) serves as the solvent for stock solutions of VP-16 and CPT. Control reactions were performed to assay for the effects of DMSO on MRC-mediated DNA replication. Drug dose-response curves for ara-CTP, CPT and VP-16 were...

P

Flavonoids of, structure of two active, 145 purification of the, F001 fraction, 141 rechromatographed F007 fraction of, 143 testing of F001 fractions from the OSU plant collection, 140 , Papovaviruses DNA replication in vitro, a multiprotein DNA polymerase mediates, 154-155 models for mammalian replicons, 1-8 papilloma, 2 papovaviruses, 1-3 polyomaviruses, 1-2, 49 Percent hydrogen (pH), 134 Permana P, 31, 175 Perrino FW, 155 Phenolic compounds, 135 Plant fractions, 135. See also Cell extracts....

Info

The 40S intermediates begin to break down as they are being formed. This is indicated by low-level protein-DNA crosslinking immediately after aphidicolin exposure. However, formation of 40S intermediates is more rapid than their breakdown so that a substantial temporary accumulation of 40S intermediates occurs. Protein-DNA crosslinks reach a maximum as nicking of the 40S intermediates is completed in the somewhat slower breakdown phase. A slower secondary breakdown occurs over the next 24 hours...

S

One dimensional agarose gel electrophoresis of SV40 DNA replication intermediates resulting from exposure to dimethyl sulfate. SV40-infected cells were exposed to the indicated concentrations of dimethyl sulfate for the last 15 min of a 30 min labeling with tritiated thymidine. The SV40 DNA in the Hirt extract supernatant was deproteinized by digestion with proteinase K 4 hr at 45 C and chloroform isopropanol extraction before electrophoresis. 1. Snapka RM. Topoisomerase inhibitors...