Multiplying by 1/KDN2 obtain phase. The partition coefficient refers to the distribution of one species, so the assay data of the solute in each phase have to be corrected for ionisation. We can use the convention that Cw is the total concentration of all species in the aqueous phase, Co is the concentration in the organic phase and Cion is the concentration of ions in the aqueous phase. If we consider the dissociation of a weak acid

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