M

The total volume V of a solution containing g1 grams of solvent and g2 grams of dry macromolecular solute is

V = volume of solute

+volume of water of hydration +volume of free solvent nrel - 1 + 2.50

Therefore,

If the particle can be assumed to be unhydrated, or if the degree of hydration can be estimated with certainty from other experimental techniques, equation (8.15) may be used to determine the asymmetry of the particle. Alternatively, if the macromolecule may be assumed to be symmetrical or its asymmetry is known from other techniques, then this equation may be used to estimate the extent of hydration of the macromolecule.

Substituting for v2 in equation (8.11) gives

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