Method III

Collodion was placed on top of a liquid which is immiscible with water and which has a specific gravity greater than collodion but less than saline. (In this case paraffin oil was used.) A small drop of hemoglobin solution was dropped about 1/2 feet from the surface of the collodion solution. This height is to give the hemoglobin droplet the neccessary force to penetrate the collodion solution. As the hemoglobin droplet passes through the collodion solution a film of collodion is attached to its surface. Due to the force of gravity, this droplet falls into the paraffin oil through the collodion layer. The purpose of the paraffin oil is to allow the collodion membrane time to set on the droplet of the hemoglobin solution since if this droplet was to enter water right away the membrane would rupture since it is still immaturely set. About 30 minutes was allowed for the droplet to remain in the paraffin oil. At

Fig. 5.

the end of this time the membrane was perfectly set, and the collodion layer together with as much as possible of the paraffin oil was removed. The remaining paraffin oil, together the hemoglobin corpuscle was then emulsified in saline, the paraffin oil having been emulsified and float to the surface leaving behind the hemoglobin corpuscle in saline.

With this method, it was successful in preparing hemoglobin corpuscles with size of about 0.2 mm in diameter. The trouble with this method being that the surface tension between collodion and paraffin oil was such that only hemoglobin droplets of appreciable size would be able to penetrate the collodion-paraffin oil interface.

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