Temperature

The range of temperatures used to maintain slices for study typically is quite narrow, lows being near room temperature (25°C) and highs closer to the temperature of

FIGURE 10.3

Cross-sectional view of a Haas-type recording chamber used for brain hippocampal slice studies.

FIGURE 10.3

Cross-sectional view of a Haas-type recording chamber used for brain hippocampal slice studies.

TABLE 10.1

Comparison Between a Composite Formula for Artificial Cerebrospinal Fluid (ACSF) Taken From Examples in the Literature and the One Used by the Authors

TABLE 10.1

Comparison Between a Composite Formula for Artificial Cerebrospinal Fluid (ACSF) Taken From Examples in the Literature and the One Used by the Authors

Composition of Artificial Cerebrospinal Fluid (ACSF) in mM

Na K Mg Ca Cl HCO3 Glu Ref.

Range Authors

143-152 2.5-6.4 1.3-2.4 1.5-2.5 127-136 24-26 4-10 10 150 3.75 2 2.5 132 26 10 15

small rodents (38 to 39°C). Our laboratory transfers the slices to the chamber at room temperature and permits them to equilibrate with the new environment for 20 min prior to slowly warming them. Many of our studies have been done at 35°C for the duration of the experiment. This is the temperature suggested by Conners and Gutnick11 to avoid possible metabolic problems in the slice, such as deficits of either oxygen or glucose, essential nutrients for brain tissue. Slice temperature is an important variable and the use of a reliable thermocouple and heater such as the temperature controller available from Medical Systems Corp., Greenvale, NY (Temperature Controller, Model TC-102) has proven reliable and easy to maintain.

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