Transfer Techniques

A small-diameter camel hair brush can be used to remove the cut slices from the knife blade of the tissue chopper or to gently tease them from the surface of the hippocampal block as it rests on the chopper platform. Some investigators place the slices in a small amount of chilled buffer (ACSF) while waiting for completion of the slicing process. They then use either a brush, medicine dropper, or pipette to transfer the slices to the recording chamber.28 We have successfully eliminated this intermediate step by taking the slices directly from the knife to the surface of the recording chamber with a fine bristled brush. We typically place about six slices in the space available within the recording chamber.

Additional slices taken from the remainder of the hippocampus are placed in a well-oxygenated holding chamber (Prechamber, Medical Systems Corp., Greenvale, NY) containing ACSF and are held at room temperature until they are used (Figure 10.2).

HIPPOCAMP AL SUCES

HIPPOCAMP AL SUCES

FIGURE 10.2

Depiction of a prechamber for maintaining hippocampal slices in artificial cerebrospinal fluid.

HOLDING CHAMBER

FIGURE 10.2

Depiction of a prechamber for maintaining hippocampal slices in artificial cerebrospinal fluid.

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