Acinetobacter calcoaceticus RAG-1 produces a potent extracellular polymeric bioemulsifier called emulsan48 which is characterized as a polyanionic amphipathic heteropolysaccharide. The heteropolysaccharide backbone consists of repeating units of trisaccharide of N- acetyl-D-galac-tosamine, N-acetylgalactosamine uronic acid and an unidentified N- acetylamino sugar.49 Removal of the protein fraction yields a product, apoemulsan, which exhibits much lower emulsifying activity on hydrophobic substrates such as n-hexadecane. One of the key proteins associated with the emulsan complex is a cell surface esterase.50

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