Learning from Viruses Highthroughput Cloning using the Gateway System to Transfer Genes without Restriction Enzymes 605

Jonathan D. Chesnut

2.1 Introduction 605

2.2 Background 606

2.3 Engineering the Lambda System to Create Gateway 609

2.4 The Gateway Reactions 610

2.5 Creating Gateway Entry Clones 611

2.6 Gateway Destination Vectors 613

2.7 Applications Enabled by Gateway Cloning 614

2.8 HTP Expression Analysis in Mammalian Cells 614

2.9 HTP Cloning and Expression in a Baculovirus System 615

2.10 Multisite Gateway 616

2.11 Creation of Entry Vectors and Three-fragment Multisite Assembly Reaction 618

2.12 Perspective 621

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