Lipid nanoparticles

Solid lipid nanoparticles (SLNs) and lipid nano-capsules (LNCs) are under investigation as drug delivery systems for brain targeting. SLNs represent an attractive colloidal drug carrier system (M ller & Lucks, 1996). SLNs are spherical particles in the nanometer range made from solid lipid(s), emulsifier(s), and water. The solid hydrophobic core is made up of solid lipids including triglycerides, partial glycerides, fatty acids, steroids, and waxes and they are stabilized by all classes of...

Gabriel A Silva

Departments of Bioengineering and Ophthalmology and Neurosciences Program, University of California, San Diego, Abstract Functionalized quantum dot nanocrystals provide an opportunity for high signal-to-noise ratio specific labeling of cells with micron-scale spatial resolution, and extend the cellular imaging toolbox available to the cellular neurobiologist. In this review we discuss previous work from our group aimed at optimizing quantum dot labeling protocols specific to neurons and neural...

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Fluorescent labeling of neurons and glia with antibody-conjugated 605-nm quantum dots. (a) Primary cortical neurons specifically labeled for -tubulin. (b, c) Primary cortical astrocytes specifically labeled for GFAP. (d, f) PC12 cells labeled for -tubulin. (e) r-MC1 neural retinal Muller glial cells specifically labeled for GFAP. (g) PC12 cells labeled for -tubulin using standard ICC. (h) Primary spinal cord astrocytes labeled for GFAP using standard ICC. (i) An example of artifactual...

Surface modification of colloidal carriers for CNS drug delivery

In order to be able to cross the BBB, the colloidal systems have to possess appropriate targeting moieties on their surface. The surface modifications of the colloidal systems that have been shown to be able to cross the BBB can be classified into four classes (the strategies marked with an asterisk have been tested in the presence of a loaded drug or genetic material) (Scheme 1). This has been the first successful approach for polymeric Np drug delivery to CNS. The presence of a surface active...

Brain drug delivery strategies via colloid carriers

In general, colloidal drug carriers include micelles, emulsions, liposomes, and NPs nano-spheres and nanocapsules . It is noteworthy that only liposomes and NPs have been largely exploited for brain drug delivery. The aim in using colloidal carriers is generally to increase the specificity toward cells or tissues, to improve the bioavailability of drugs by increasing their diffusion through biological membranes, and or to protect them against enzyme inactivation. Moreover, the colloidal systems...