Due the large dynamic range of tissue sizes required for analysis in the present study (average total protein 4.5gm for adult cortex to 30mg for P1 optic nerve, and 0.15mg for neurones to 0.5 gm for oligodendrocytes), it was important that the aspartoacylase assay was not only linear between different varieties of biological tissues but also consistent over the extended time course of the study. We therefore optimised the assay for the very small tissue size available for some samples such as optic nerves and cellular preparations. Assaying control samples on a regular basis enabled us to monitor the long-term consistency of the enzyme assay. Thus, all differences observed between samples represent valid differences in aspartoacylase activity.
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