Cell Harvesting Extraction and Preparation of Samples

All cells were continuously cultured until sufficient material was available for highresolution NMR (typically 107-108 cells for a sample) and enzyme assays. Cells were always harvested 24 hours after a final medium change for all cell types. Cells were washed 2 times with 25ml phosphate buffered saline while still attached to the culture plastics. The cells were then scraped off the plastic surface using silicon rubber cell scrapers. The cells were spun at 2000g in a microfuge for <1 min. The supernatant was removed and the pellet frozen immediately in liquid nitrogen (for further details see Bhakoo et al.49)

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