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200 kD

45 kD

Asp-NAT fraction by centrifugation (22,000g for 30 min). Asp-NAT activity was measured by the TLC-radiometric assay previously described.12 The results are given in Figure 4. While CHAPS was the most effective detergent, Triton X-100 and laurylmaltoside were almost as effective. Deoxycholate was very low in effectiveness and CTAB was least effective.

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