Naa Naag and GAD67 Expression in Forebrain and Midbrain

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Figure 3 shows the expression of NAAG, NAA and glutatmic acid decarboxylase (GAD67) in rat neocortex and hippocampus as compared with midbrain structures. NAAG was distributed differentially, with midbrain and hindbrain having substantially higher levels than telencephalic cortical structures (Figure 3A). NAA distribution was relatively ubiquitous, and similar levels were observed in forebrain and hindbrain (Figure 3B).

Figure 3. NAAG, NAA and GAD67 expression in rat forebrain and midbrain. NAAG expression is relatively low in neocortex and hippocampus as compared with the midbrain tectum, tegmentum and hindbrain (A). NAA immunoreactivity, in contrast, is expressed differentially in different neuronal groups, but in general, high levels are found throughout the CNS (B). GAD67 expression is extremely varied, as was the case with NAAG, with high levels in hippocampal granule cell and pyramidal cell layers, and very high expression in the reticular part of the substantia nigra and tectum (C). Bar = 400^m.

Figure 3. NAAG, NAA and GAD67 expression in rat forebrain and midbrain. NAAG expression is relatively low in neocortex and hippocampus as compared with the midbrain tectum, tegmentum and hindbrain (A). NAA immunoreactivity, in contrast, is expressed differentially in different neuronal groups, but in general, high levels are found throughout the CNS (B). GAD67 expression is extremely varied, as was the case with NAAG, with high levels in hippocampal granule cell and pyramidal cell layers, and very high expression in the reticular part of the substantia nigra and tectum (C). Bar = 400^m.

Figure 4. NAAG (A) and NAA (B) are both extensively distributed throughout the brainstem. NAAG expression is very high in the brainstem and deep cerebellar nuclei (DCN), but was much more limited in the cerebellar cortex (CC). In contrast, NAA expression was very high in the CC. Both NAAG and NAA were expressed in many cell groups throughout the brainstem, such as the vestibular nuclei (VN). Many NAAG and NAA-containing axons are present throughout the cerebellar white matter. Bar = 400^m.

Figure 4. NAAG (A) and NAA (B) are both extensively distributed throughout the brainstem. NAAG expression is very high in the brainstem and deep cerebellar nuclei (DCN), but was much more limited in the cerebellar cortex (CC). In contrast, NAA expression was very high in the CC. Both NAAG and NAA were expressed in many cell groups throughout the brainstem, such as the vestibular nuclei (VN). Many NAAG and NAA-containing axons are present throughout the cerebellar white matter. Bar = 400^m.

GAD67 expression was somewhat higher in many midbrain structures than in most cortical layers, but the increase along the rostro-caudal axis of the CNS was not as apparent as was the case with NAAG expression (Figure 3C).

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