Preparation of Cortical Neurons

Cortices from embryonic day 14-16 rats were used to harvest the neurons.49 The cells were cultured in DMEM-BS supplemented with 10 ng/ml of basic fibroblast growth factor, and 50 ng/ml of nerve growth factor (Roche Diagnostics Ltd, Lewes, UK). The growth factors were added every second day to improve cell survival. Every second day, cells were pulsed with 20 mm cytosine arabinoside to prevent glial cell proliferation. This procedure routinely produced neuronal cultures of 95% purity as assessed by immunostaining with RT97.50 Cells were harvested after 7 days of culture.

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