W

Wallerian degeneration, 207-208, 211 Water content of brain, 115, 122, 189 Water-soluble carbodiimide, EDAC, 7 Water-suppressed spectrum, 189 WBNAA see Whole Brain NAA WCST see Wisconsin Card Sorting test Whole brain lipid analysis, 132 Whole brain NAA, 211 Whole brain ratio of, 213 Whole-brain PET data, 252 Wisconsin Card Sorting test, 234

UFLGTCVC see UFL Gene Vector Core Xenopus laevis oocytes, 69

UFL Gene Vector Core, 84 X-linked leukodystrophy, 357

UMNs see Upper motor neurons

UMN pathology of, 280 Z

Unsuppressed tissue water signal of, 189

Upper motor neurons, 279 Zidovudine treatment of AIDS, 282

Urea cycle system, 59

DIG Sense DIG Anti-Sense

Figure 1. Characterization of primary cultures of neural cells from newborn rat cortex for expression of ASPA mRNA, enzyme activity and co-immunostaining of ASPA protein with neural cells markers. The in situ detection of ASPA mRNA in oligodendrocytes cells in cultures mixed glia probed with sense (A) and anti-sense (B) ASPA digoxigenin labeled probes. The oligodendrocyte cells show dark staining (arrows) on the bed layer of unstained astrocytes. Optimal ASPA enzyme activity (mU/mg protein) can be seen in culture of oligodendrocytes (C) and about 29% ASPA activity can also be seen in mixed glial cultures. The pure cultures of astrocytes and cortical neurons exhibit insignificant level of ASPA activity. Immunostaining of mixed glial cultures from normal rat brain shows a co-localization of ASPA (red) with oligodendrocyte cell specific markers A2B5 (D) and CNP (F), both green, or exhibiting a yellow staining for co-expression. The immunostaining of ASPA with GFAP (green), an astrocyte cell specific marker shows a discrete localization of the two markers (H). (epifluorescence images D, F, H; Phase image E, G, I).

Figure 1. Characterization of primary cultures of neural cells from newborn rat cortex for expression of ASPA mRNA, enzyme activity and co-immunostaining of ASPA protein with neural cells markers. The in situ detection of ASPA mRNA in oligodendrocytes cells in cultures mixed glia probed with sense (A) and anti-sense (B) ASPA digoxigenin labeled probes. The oligodendrocyte cells show dark staining (arrows) on the bed layer of unstained astrocytes. Optimal ASPA enzyme activity (mU/mg protein) can be seen in culture of oligodendrocytes (C) and about 29% ASPA activity can also be seen in mixed glial cultures. The pure cultures of astrocytes and cortical neurons exhibit insignificant level of ASPA activity. Immunostaining of mixed glial cultures from normal rat brain shows a co-localization of ASPA (red) with oligodendrocyte cell specific markers A2B5 (D) and CNP (F), both green, or exhibiting a yellow staining for co-expression. The immunostaining of ASPA with GFAP (green), an astrocyte cell specific marker shows a discrete localization of the two markers (H). (epifluorescence images D, F, H; Phase image E, G, I).

Figure 2. Immunocytochemistry of mixed glia cultures from ASPA wt and KO mice cerebral cortex. The immunostaining of markers for progenitor and mature oligodendrocytes were carried out in 6 and 15d-old cultures. The immunostaining for marker NG2 (green) and GD3 (Red; A and B), A2B5> (red) and GPDH (green; C and D), and O4 (red; E and F) showed the status of oligodendrocytes, while GFAP (green; E and F), the marker for astrocyte shows no co-localization with oligodendrocyte marker O4. A delay in MBP (green) and PLP (red) expressing oligodendrocyte cells was detected in 15day old ASPA KO mice cortical cultures (G, I and K). The double immunostaining of MBP astrocyte, showed a discrete staining for ASPA (red) and GFAP (green) in mixed glial cultures (Fig. 1G and panel H as phase image).

Figure 2. Immunocytochemistry of mixed glia cultures from ASPA wt and KO mice cerebral cortex. The immunostaining of markers for progenitor and mature oligodendrocytes were carried out in 6 and 15d-old cultures. The immunostaining for marker NG2 (green) and GD3 (Red; A and B), A2B5> (red) and GPDH (green; C and D), and O4 (red; E and F) showed the status of oligodendrocytes, while GFAP (green; E and F), the marker for astrocyte shows no co-localization with oligodendrocyte marker O4. A delay in MBP (green) and PLP (red) expressing oligodendrocyte cells was detected in 15day old ASPA KO mice cortical cultures (G, I and K). The double immunostaining of MBP astrocyte, showed a discrete staining for ASPA (red) and GFAP (green) in mixed glial cultures (Fig. 1G and panel H as phase image).

Figure 2. Spectroscopic Imaging (SI) of child with TBI maximally affecting the left cerebral hemisphere.
Figure 3 (left panel). Voxel Based Morphometry (VBM) analysis of GM cluster volumes covarying with posterior left hemisphere white matter NAA concentration.

Figure 4 (right panel). Voxel Based Morphometry (VBM) analysis of GM cluster volumes covarying with left hemisphere frontal white matter NAA concentration.

3 56

0 0

Post a comment