Reagents and Chemicals
Affi-Gel 15
Trypsin
Trypsin inhibitor Yeast extract
Hydroxyapatite Nutrient broth Sephacryl S-300 TRITC-anti rabbit IgG
Agar Noble CM-Sepahdex A-50
Materials
Cooked meat medium
DEAE-Sepahdex A-50 Ficoll 400
Supplier
Bio-Rad
Difco laboratoires
Pharmacia
Difco Laboratories
Pharmacia
Pharmacia
Bio-Rad
Difco Laboratories
Pharmacia
Biosource
International
(Tago Products)
Sigma
Sigma
Difco Laboratories
T 7409 T 9003 527-00305
153-6051
526-00054
17-0220-01
529-00804
17-0180-01
17-0400-01
157-0080
523-00483
17-0599-01
ALI-4406
Cat-No.
References
Bengston IA (1922): Preliminary note on a toxin-producing anaerobe isolated from the larvae of Lucilia caesar. Public Health Rep. 37: 164-170.
Iwasaki M, Ohishi I, Sakaguchi G (1980): Evidence that botulinum C2 toxin has two dissimilar components. Infect. Immun. 29: 390-394.
Jansen BC (1971): The toxic antigenic factors produced by Clostridium botulinum types C and D. Onderstepoort J. Vet. Res. 38: 93-98.
Mason JH, Robinson EM (1935): The antigenic components of the toxins of C. botulinum types C and D. Onderstepoort J. Vet. Sei. Anim. Indus. 5: 65-75.
Miyake M, Ohishi I (1987): Reponse of tissue-cultured cynomolgus monkey kidney cells to botulinum C2 toxin. Microbial Pathogenesis, 3: 279-286.
Ohishi I, Iwasaki M, Sakaguchi G (1980): Purification and characterization of two components of botulinum C2 toxin. Infect. Immun. 30: 668-673.
Ohishi I, Iwasaki M, Sakaguchi G (1980): Vascular permeability activity of botulinum C2 toxin elicited by cooperation of two dissimilar protein components. Infect. Immun. 31: 890-895.
Ohishi I, Sakaguchi G (1982): Production of C2 toxin by Clostridium botulinum types C and D as determined by its vascular permeability activity. Infect. Immun.
Ohishi I (1983): Response of mouse intestinal loop to botulinum C2 toxin: en-terotoxic activity induced by cooperation of nonlinked protein components. Infect, immun. 40: 691-695.
Ohishi I, Tsuyama S (1986): ADP-ribosylation of nonmuscle actin with component I of C2 toxin. Biochem. Biophys. Res. Commun. 136: 802-806.
Ohishi I (1986): NAD-glycohydrolase activity of botulinum C2 toxin: a possible role of component I in the mode of action of the toxin. J. Biochem. 100: 407-413.
Ohishi I (1987): Activation of botulinum C2 toxin by trypsin. Infect. Immun. 55: 1461-1465.
Ohishi I, Morikawa Y, Baba T (1990): ADP-ribosylation of nonmuscle actin by component I of botulinum C2 toxin inactivates the ability to interact with unmodified actin. J. Biochem. 107: 420-425.
Ohishi I (1992): Visualizations of binding and internalization of two nonlinked protein components of botulinum C2 toxin in tissue culture cells. 60: 4648-4655.
Bacterial Toxins
Kletus AKtories
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Anti-Aging Report
When people generally think about anti-aging, they tend to think about the visible signs of wear and tear, those tell-tale wrinkles, age spots and their developing jowls. No-one wants to get old, let alone feel and look older than their years and anti-aging treatments are becoming so sought after by both men and women that the skincare market is colossal, but what really works?
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