Reagents and Chemicals

Affi-Gel 15

Trypsin

Trypsin inhibitor Yeast extract

Hydroxyapatite Nutrient broth Sephacryl S-300 TRITC-anti rabbit IgG

Agar Noble CM-Sepahdex A-50

Materials

Cooked meat medium

DEAE-Sepahdex A-50 Ficoll 400

Supplier

Bio-Rad

Difco laboratoires

Pharmacia

Difco Laboratories

Pharmacia

Pharmacia

Bio-Rad

Difco Laboratories

Pharmacia

Biosource

International

(Tago Products)

Sigma

Sigma

Difco Laboratories

T 7409 T 9003 527-00305

153-6051

526-00054

17-0220-01

529-00804

17-0180-01

17-0400-01

157-0080

523-00483

17-0599-01

ALI-4406

Cat-No.

References

Bengston IA (1922): Preliminary note on a toxin-producing anaerobe isolated from the larvae of Lucilia caesar. Public Health Rep. 37: 164-170.

Iwasaki M, Ohishi I, Sakaguchi G (1980): Evidence that botulinum C2 toxin has two dissimilar components. Infect. Immun. 29: 390-394.

Jansen BC (1971): The toxic antigenic factors produced by Clostridium botulinum types C and D. Onderstepoort J. Vet. Res. 38: 93-98.

Mason JH, Robinson EM (1935): The antigenic components of the toxins of C. botulinum types C and D. Onderstepoort J. Vet. Sei. Anim. Indus. 5: 65-75.

Miyake M, Ohishi I (1987): Reponse of tissue-cultured cynomolgus monkey kidney cells to botulinum C2 toxin. Microbial Pathogenesis, 3: 279-286.

Ohishi I, Iwasaki M, Sakaguchi G (1980): Purification and characterization of two components of botulinum C2 toxin. Infect. Immun. 30: 668-673.

Ohishi I, Iwasaki M, Sakaguchi G (1980): Vascular permeability activity of botulinum C2 toxin elicited by cooperation of two dissimilar protein components. Infect. Immun. 31: 890-895.

Ohishi I, Sakaguchi G (1982): Production of C2 toxin by Clostridium botulinum types C and D as determined by its vascular permeability activity. Infect. Immun.

Ohishi I (1983): Response of mouse intestinal loop to botulinum C2 toxin: en-terotoxic activity induced by cooperation of nonlinked protein components. Infect, immun. 40: 691-695.

Ohishi I, Tsuyama S (1986): ADP-ribosylation of nonmuscle actin with component I of C2 toxin. Biochem. Biophys. Res. Commun. 136: 802-806.

Ohishi I (1986): NAD-glycohydrolase activity of botulinum C2 toxin: a possible role of component I in the mode of action of the toxin. J. Biochem. 100: 407-413.

Ohishi I (1987): Activation of botulinum C2 toxin by trypsin. Infect. Immun. 55: 1461-1465.

Ohishi I, Morikawa Y, Baba T (1990): ADP-ribosylation of nonmuscle actin by component I of botulinum C2 toxin inactivates the ability to interact with unmodified actin. J. Biochem. 107: 420-425.

Ohishi I (1992): Visualizations of binding and internalization of two nonlinked protein components of botulinum C2 toxin in tissue culture cells. 60: 4648-4655.

Bacterial Toxins

Kletus AKtories

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