Ephedra Ma Huang And Related Drugs

Ma huang (ephedra) is commonly found in herbal weight loss products that are often referred to herbal fen-phen. Some weight loss clinics and herbal outlets promote 'Herbal fen-phen' as an alternative to fenfluramine, the prescription drug that has been withdrawn from the market due to toxicity. Herbal fen-phen products sometimes contain St John's wort and are sold as 'herbal prozac'. Ephedra-containing products are also marketed as decongestants, bronchodilators and stimulants. Other promoted...

Lcms Analysis Of Natural Androgens In Human Samples

Table 2.1 summarizes publications describing the measurement of natural anabolic steroids as standards or in human samples. Listed are analytes, samples, sample preparation, LC conditions, MS-MS conditions, whether the assay is quantitative or qualitative and references. In anticipation of potential studies involving endogenous steroids, including neuroster-oids, or exogenous steroids, or metabolites, Ma and Kim (1997) reported results obtained by LC-MS analysis by APCI or ESI of 29 steroid...

Herbal Supplements With Digoxinlike Immunoreactivity

Several herbal supplements, including several Chinese medicines, have digoxin-like immu-noreactivity because some components have structural similarity to digoxin. Digoxin immunoassays available commercially may use either a monoclonal antibody specific to di-goxin or polyclonal antibodies. In general, assays that employ polyclonal antibodies against digoxin, such as the fluorescence polarization immunoassay (rabbit polyclonal antibody) and microparticle enzyme immunoassay (both marketed by...

References

Herbal use in ED patients. Acad Emerg Med 2001 8 450. 2. Klepser TB, Klepser ME. Unsafe and potentially safe herbal therapies. Am J Health Syst Pharm 1999 56 125-141. 3. Raffa R. Screen of receptor and uptake site activity of hypericin components of St John's wort reveal ct receptor binding. Life Sci 1998 62 265-270. 4. Johne A, Brockmoller J, Bauer S, et al. Pharmacokinetic interaction of digoxin with an herbal extract from St John's wort (Hypericum perforatum)....

HPLC testing for CDG

Using the improved HPLC method,31 sera from CDG-I patients showed increased relative amounts of disialo- and asialotransferrin and concomitant reductions in tetrasialotransferrin (Figure 6.4). Patients with the CDG-Ia subtype showed the highest amounts of disialo-(range, 19-42 ) and asialotransferrin (3.4-26 ), whereas the patients with type Ib and Ig had less asialotransferrin (< 3 ). After the CDG-Ib patient had been treated with low-dose mannose,58 the abnormal transferrin pattern...

Ghb Interpretation Issues And Postmortem Production

The most difficult aspect of GHB analysis is interpretation of the numbers that are generated. Helrich et al. (1964) correlated blood concentrations of GHB with state of consciousness in 16 adult human patients (Table 11.4). This study revealed that GHB blood concentrations as high as 99 mg mL-1 could be achieved with the patient still displaying an 'awake' state, the subject spontaneously coming in and out of consciousness characterized a light sleep state. Subjects in the medium sleep state...

Carbohydratedeficient Transferrin

The abnormal glycoform pattern of serum transferrin that occurs as a result of prolonged heavy alcohol consumption, CDT, has emerged as a useful biochemical marker for identifying persons chronically abusing alcohol and for monitoring abstinence from alcohol during treatment.4,9 Regular high alcohol intake averaging at least 50-80 g of ethanol per day for two or more weeks typically results in an altered transferrin glycoform profile. The biological mechanism by which alcohol causes changes in...

St Johns Wort

St John's wort is a very popular herbal antidepressant. The most widely available St John's wort preparation in the USA comes from the dried alcoholic extract of hypercian, a perennial aromatic shrub with bright yellow flowers that bloom from June to September. The flowers are believed to be most abundant and brightest around 24 June, the day traditionally believed to be the birthday of John the Baptist. Therefore, the name St John's wort became popular. The German Commission E monograph...

Info

Measurement of Plasma l-DOPA and l-Tyrosine by High-Performance Liquid Chromatography as a Tumor Marker in Melanoma Thierry Le Bricon,1 Sabine Letellier,1,2 Konstantin Stoitchkov,3,4 and Jean-Pierre Garnier1,2 1 Laboratoire de Biochimie A H pital Saint-Louis AP-HP , 1 Avenue Claude Vellefaux, 75010 Paris, France Facult des Sciences Pharmaceutiques et Biologiques, Universit Paris V, 4 Avenue de l'Observatoire, 75270 Paris, France 3Department of Dermatology, National Center of Oncology,...

Analysis For Ghb Gbl And 14bd

GHB does not exist in a static state, even outside the body. In solution, it exists in a dynamic state in equilibrium with its lactone, GBL Figure 11.2 . The ratio of the two forms is dependent on the pH of the matrix or the type of matrix containing the GHB. For example, in blood, the GHB acid form predominates because the lactonase enzyme converts any of the lactone to the acid. However, if the GHB is in a matrix that does not contain this enzyme, such as urine, water or juice, the two forms...

Lcms Analysis Of Synthetic Steroids Or Animal Samples

In veterinary medicine, boldenone, a synthetic anabolic steroid Figure 2.1 , is commercially available, hence it is a concern in the horseracing industry. Pu et al. 2004 used ion-trap LC-MS analysis to detect boldenone conjugates sulfate and glucuronide and their 17-epimers in horse urine after intramuscular administration of boldenone undecylenate. Soon afterwards, Ho et al. 2004 reported the occurrence of endogenous boldenone sulfate in the urine of uncastrated male horses, and quantitated it...

Analysis

Neuromuscular blockers can be analyzed using HPLC with fluorescence, ultraviolet UV , or electrochemical detection ECD . As an alternative to HPLC, GC with nitrogen-phosphorus detection NPD and LC coupled with electrospray ionization tandem mass spectrometry ESI-MS-MS have also been investigated as viable techniques to measure neuromuscular blockers Table 10.2 . Regardless of the chromatographic technique, NMBAs are purified from biological tissues and fluids by both liquid-liquid extractions...