The everted gut technique has been in use from as early as the 1950s (Wilson and Wiseman, 1954) in the transport study of sugars and amino acids. Over the years modifications such as use of physiologically relevant tissue culture media, means to maintain constant oxygenation and improved incubation apparatus have all contributed to increased viability of ex vivo tissues leading to better predictability with everted tissue studies. This model is ideal for studying the absorption mechanism of drugs since both the passive and active transport can be studied. With the recent interest in the field P-glycoprotein activity in the gut, this model may be used to evaluate the role of efflux transporters in the intestinal absorption of drugs by comparing the transport kinetics of drug in the absence and presence of P-glycoprotein inhibitors or substrates. The everted gut model has an additional analytical advantage over other in vitro models because the sample volume on the serosal side is relatively small and drugs accumulate faster. Some of the disadvantages are the lack of active blood and nerve supply which can lead to a rapid loss of viability. In addition everting the intestinal tissue can lead to morphological damage causing misleading results.
Was this article helpful?