Applications And Examples In Drug Development And Diagnostics

Whether one uses planar arrays or microsphere arrays, the resulting quantitative, multiplexed data are then mined for distinctive patterns of biomarkers that indicate disease, drug toxicity or efficacy, or some other useful biochemical phenotype. We will not discuss the data mining techniques available but simply describe several examples where data mining of these complex data sets has revealed meaningful biomarker patterns. Typically, data mining is first done using traditional statistics to identify those analytes whose values are significantly different between two experimental groups. This is often done by determining mean analyte values and applying Student's ¿-tests to determine p- values of significance. The next step is often to use more complex multi-variate analysis tools, such as principal component analysis, for pattern recognition.

The reports of IB monitoring cited most often are related to inflammatory disease. Asthma, rheumatoid arthritis (RA), multiple sclerosis (MS), systemic lupus erythematosus (SLE), chronic obstructive pulmonary disease (COPD), and psoriasis (PS) are thought of as classic inflammatory diseases. In recent years, a host of other diseases have been found to have a significant inflammatory component. These include cardiovascular disease (CVD), central nervous system diseases such as Alzheimer disease, most infectious diseases, and various cancers [6] . IBs are thus very useful for quantifying, characterizing, and monitoring the inflammatory and anti-inflammatory components of a disease in addition to therapeutic benefits during drug trials.

Measurement of inflammatory markers is a popular application of multiplexed profiling in drug development and diagnostic efforts. The duration and pattern of cytokine and chemokine expression in serum or plasma have been the most common uses of multiplexed immunoassays. Common inflammatory mediators are often cited (e.g., TNFa, IL-1, IL-6, IL-8, MIP-1) due to the large set of publications describing the association of these classical inflammatory markers with numerous diseases, conditions, and drug treatments. Although important, other IBs that are related directly or indirectly to the disease and/ or therapy in question will often add significant prognostic value to biomarker patterning efforts.

Project Management Made Easy

Project Management Made Easy

What you need to know about… Project Management Made Easy! Project management consists of more than just a large building project and can encompass small projects as well. No matter what the size of your project, you need to have some sort of project management. How you manage your project has everything to do with its outcome.

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