Info

RNA 125-mer MW 38,641 Da, Intensity - Ion Counts, Bio-Gel P6 Resin (1,000-6,000 Fractionation Range), RNA/Drug Ratios 10/300 mM/mM, Incubation Buffer 20 mM HEPES, 1 mM DTT, pH 7.5, Centrifuged 3 min at 775xg

RNA 125-mer MW 38,641 Da, Intensity - Ion Counts, Bio-Gel P6 Resin (1,000-6,000 Fractionation Range), RNA/Drug Ratios 10/300 mM/mM, Incubation Buffer 20 mM HEPES, 1 mM DTT, pH 7.5, Centrifuged 3 min at 775xg

2.3.1.4 Amgen Secondary Screens

Hits obtained in the primary screens of 80 compound mixtures (see Section 2.3.2.2) were rerun in the secondary screens as singletons.

2.3.1.5 Novartis Secondary Screens

Novartis GPC spin column/HPLC ESI-MS secondary screens were run in conjunction with their primary screens to confirm the hits found in the primary screen. The method used was identical to that of the GPC spin column/HPLC ESI-MS primary screen, as described in Section 2.3.2.3, except that the hits were run as singletons in triplicate.

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