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The GPC spin column/ESI-MS technology has been utilized for primary and secondary screening of drug candidates. For primary screens, large compound libraries are assayed in a high throughput mode to find new non-covalent binders to a target protein. For secondary screens, small subsets of libraries containing compounds believed to bind to a target protein, generally from data obtained using any drug screening technology, are evaluated by the GPC spin column/ ESI-MS method to confirm or deny non-covalent binding.

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