Contents

4.1 Introduction 59

4.2 Assay Development for G Protein-Coupled Receptors 60

4.2.1 Biology of G Protein-Coupled Receptors 60

4.2.2 Non-Functional and Biochemical Assays for GPCRs 61

4.2.3 Development of Scintillation Proximity Assay for GPR23 62

4.2.4 Functional Cell-Based Assays for GPCRs 65

4.2.5 Development of Reporter Gene Assay for Calcitonin Receptor 67

4.3 Assay Development for Ion Channels 69

4.3.1 Biology of Ion Channels 69

4.3.2 Voltage-Clamp-Based Assays for Ion Channels 71

4.3.3 Development of Automated Voltage-Clamp Assay for KV1.3 73

Acknowledgments 79

References 79

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