Immunological Methods

Pfarr and co-workers have recently described a technique that renders possible immunodetection of 5mC on human chromosome metaphase spreads.81 A monoclonal antibody tagged with fluorescent dye is used to image the chromosomes after they have been previously stained by the Giemsa method. The technique can be used to obtain a fast and global overview of changes in genomic methylation patterns during the development of various types of cancer. Potential applications of the technique include characterization of disease-related methylation patterns on a genomic scale for diagnostic purposes and monitoring of patient responsiveness to therapy.

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