Reporter Assays for Gpcr Hts

In addition to direct measurements of second messengers, reporter gene assays can be used to detect the consequence of changes in second messengers [21-23]. These HTS assays employ constructs consisting of second messenger response elements such as the cAMP response element (CRE) or the calcium-sensitive activator protein 1 (API) or nuclear factor of activated T cell (NFAT) elements linked to genes that encode for enzymes, such as luciferase or p lactamase, which act to catalyze the formation of luminescent or fluorescent products. Like the photoprotein assays, this technology is highly sensitive to changes in GPCR-mediated changes in second messengers and is easily adapted to HTS format.

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