In this chapter, we established the possibilities of using roGFPs as real-time redox reporters in soil-grown plants. With the use of specific cell promoters, it is possible to target distinct cell compartments and tissues and monitor changes in GSH redox state to determine the effects of ROS on specific cellular components. This would remove the need for confocal microscopy, which would limit the size and type of plants that could be used (13). The roGFP1-R12 is a good starting point to develop an intact plant system, as it encompasses several advantages over other variants of GFP, namely dual excitation peaks allowing ratiomet-ric measurements, insensitivity to pH and halide ions, increased response times enabling real time measurements, and appropriate emission wavelengths allowing detection in green leaves. With the addition of an alteration to prevent mRNA splicing in Arabi-dopsis, roGFP1-R12 is an ideal candidate for developing a redox reporter system in whole plants.


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