Fresh 1 day 1 week 1 month 3 month

Freezing time

Fig. 2.6. Effect of sample freezing on red blood cell-associated nitrite and hemoglobin-bound nitric oxide (top) and red blood cell hemoglobin-bound NO (bottom) measured with tri-iodide with added K3FeNi(CN)6 in conjunction with chemiluminescence. Fresh represents snap frozen and immediately measured. Values presented as mean ± SEM (n = 5); *p < 0.05; **p < 0.01. Repeat-measures analysis of variance.

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