Fig. 11.2. Sypro Ruby-stained gels (A) and 2D-Oxyblots (B). Proteins spots showed a significant increase in protein carbonyl immunoreactivity, after normalization of the carbonyl intensities to the protein content are numbered on both gel (A) and blot (B). (1) Dihydropyrimidinase-related protein 2 (DRP-2); (2) alpha enolase; (3) gamma-soluble NSF attachment protein; (4) ubiquitin carboxy-terminal hydrolase L-1 (UCH L-1); (5) Peptidyl-prolyl cis-trans isomeraseA (Pin 1); (6) triose phosphate isomerase (TPI); (7) Phosphoglycerate mutase 1 (PGM1); and (8) carbonic anhydrase 2 (CA 2).

and submitted for mass spectrometry for mass analysis, finally detected using a peptide search database.

Was this article helpful?

0 0

Post a comment