Fig. 7.1 Hydrogen peroxide (H2O2) inhibition of GAPDH activity is reversible. GAPDH was pretreated with H2O2 at different concentrations before the assay (a). After GAPDH had been treated with H2O2 for 5 min, 10 mM dithiothreitol (DTT) or reduced glutathione (GSH) was added and rates calculated after a further 10 min (b).
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