0mM H2O2 0.1mM H2O2 0.1mM+DTT 0.1mM+GSH

Fig. 7.1 Hydrogen peroxide (H2O2) inhibition of GAPDH activity is reversible. GAPDH was pretreated with H2O2 at different concentrations before the assay (a). After GAPDH had been treated with H2O2 for 5 min, 10 mM dithiothreitol (DTT) or reduced glutathione (GSH) was added and rates calculated after a further 10 min (b).

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