Fig. 10.1. Electrospray mass spectrometry analysis of hBCATm samples in the absence (A) or presence (B) of DTNB treatment. Human BCATm (control) and the DTNB-labeled protein were dialyzed in 10 mM ammonium bicarbonate. The samples were then prepared for mass spectrometry analysis as described in Sect. 3.3. Shown are transformed data, which present the relative abundance of masses for control hBCATm (A) and hBCATm incubated with DTNB (B). The major peaks shown in (A) (41 736) and (B) (41 731 and 41 968) were accompanied by minor peaks at greater molecular weights in each case, which may represent adventitious oxidation with prolonged incubation times. Two minor species in (B) likely represent proteins labeled with two TNB moieties (42 124) and a corresponding oxidized species (42 164). Reproduced from ref. (12). With permission from American Chemical Society.
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