1. AT3 medium: Murashige and Skoog medium supplemented with 3% sucrose, 0.5 mg/L NAA and 0.05 mg/L kinetin, pH 5.5.

2. Murashige and Skoog media (Sigma, UK, cat. no. M5515).

3. Extraction buffer: 50 mM Tris-HCl, pH 7.5, with complete protease inhibitor cocktail.

4. Equilibration solution: 50 mM Tris-HCl, pH 8.8; 6 M urea; 30% glycerol; 2% sodium dodecyl sulfate (SDS); 0.025% bromophenol blue.

5. Protease inhibitor cocktail tablets (Roche).

6. 5'-iodoacetamide fluorescein (5'-IAF) made as a stock solution fresh (for example, 10 mM stock in dimethyl sulfoxide).

7. Two-dimensional electrophoresis solutions and reagents: all PlusOne reagents (GE Healthcare, UK).

8. H2O2 solution (30% v/v; Sigma-Aldrich Co., Ltd., Dorset UK).

9. Stomatal bioassay buffer: 10 mM MES; 5 mM KCl; 100 pM CaCl2, pH 6.15; with 1 M Tris-HCl, pH 7.5.

10. Levington's F2 compost with sand.

11. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) assay buffer: 100 mM Tris-HCl, pH 8; 10 mM MgCl2; 0.2 mM NADH; 5 mM ATP; 2 mM phosphoglyceric acid.

12. Isolated GAPDH enzyme, for example, from rabbit muscle (Sigma).

13. MES/KCl buffer: 5 mM KCl, 10 mM MES, 50 pM CaCl2, pH 6.15.

14. Seed sterilizing solution: 10% bleach, 0.1% SDS.

Appetite Antidote

Appetite Antidote

Discover How You Can Free Yourself FromĀ  Uncontrolled Habits And Get Your Eating Under Control Once And For All! This Book Is One Of The Most Valuable Resources In The World When It Comes To Ways To Reclaime Your Rightful Body. Sound eating isn't about rigid nutrition doctrines, staying unrealistically skinny, or depriving yourself of the foods you adore.

Get My Free Ebook

Post a comment