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Fig. 13.4. 2D-gel electrophoresis of reduced and oxidized protein-thiols differentially labeled with fluorescent dyes of distinct emission wavelengths, as described in Sect. 3.2.2. Reduced thiols were first labeled with the NEM derivative DYE680 (red). Then, after their reduction by DTT, oxidized thiols were labeled with DYE780 (green). Red and green signal overlap appears in yellow. The gel on the left corresponds to untreated cells. Pdi1 and Sod1 are here fully oxidized and appear therefore in green. The gel on the right corresponds to cells treated with 1 mM of H2O2 during 2 min. The green signals which appeared on the right gel correspond to protein-thiols oxidized by H2O2 (See Color Plates).

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