Cellular Aspects of CD26DPPIV Role in Immunity

While CD26 expression is enhanced following activation of resting T cells, CD4+CD26high T cells respond maximally to recall antigens such as TT 3 . Cross-linking of CD26 and CD3 with solid-phase immobilized mAbs can induce T cell costimulation and IL-2 production by CD26+T cells 2,3 . In addition, anti-CD26 antibody treatment of T cells enhances Tyr phosphory-lation of signaling molecules such as CD3C and p56lck 7,113 . High CD26 cell-surface expression is correlated with the production of...

Babce

Chromosomal location of HLA genes associated with ARF RHD. HLA genes are located on human chromosome 6 and are represented by black boxes. Non-HLA genes are represented by gray boxes. Loci associated with ARF RHD are showed with arrows HLA-DRB4, HLA-DRB1, HLA-DQB1, HLA-DQA1 genes. The alleles HLA-DR1, 2, 3, 4, 5(11), 6, 7, and DR53 (HLA-DRB1 and HLA-DRB4 genes, respectively) were defined by serology. HLA-DQA1*0104, *0201, *0301, *0501, and HLA-DQB1 *0301, *0302, *0401, *0501 alleles...

Malditofms

The MALDI process can be separated into three fundamental steps of analyte matrix preparation, desorption of the upper matrix monolayers, and ionization ofthe analyte molecules. Successful MALDI involves incorporation of analyte molecules between the matrix molecules, either in the bulk or within the crystal surface, and separation of the analyte molecules from each other. There is no single MALDI matrix or protocol that is suited to all analytical questions, and a proper choice of both is...

Contributors

Numbers in parentheses indicate the pages on which the authors' contributions begin. Dibyajyoti Banerjee 139 , Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh, India Chiara Benedetto 85 , Department of Obstetrics and Gynaecology, University of Torino, Torino, Italy Rajasri Bhattacharyya 139 , Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh,...

Molecular Aspects of CD26DPPIV Role in Immunity

Human CD26 is a 110-kDa cell-surface glycoprotein that belongs to the serine protease family, which is characterized by the Ser-Asp-His catalytic triad in the C-terminal region. The primary structure of the enzyme was first determined by cloning and sequencing of the cDNA of rat DPPIV in 1989 25 . For human DPPIV, the complete cDNA and derived amino acid sequences were published by our group and others in 1992 2,26,27 . The human DPPIV gene is located on chromosome 2 locus 2q24.3, spans 70 kb,...

Info

Powerful and widely used genotyping technologies. The method offers great flexibility in assay design and enables highly accurate genotyping at high sample throughput. Different strategies for allele discrimination and quantification have been combined with MALDI hybridization, ligation, cleavage, and primer extension . Approaches based on primer extension have become the most popular applications. This combination enables rapid and reliable multiplexing of SNPs and other common variants, and...

Contents

xiii Use of Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry for Multiplex Genotyping 3. Genotyping Using Mass 5. Multiplex Genotyping by Rheumatic Heart Disease Mediation by Complex Immune Events L. Guilherme, K. F. KOhler, and J. Kalil 2. Rheumatic Heart 4. Genetic 5. Immune Mechanisms Involved in RHD 6. Rheumatic Heart Disease Dipeptidyl Peptidase in Autoimmune Pathophysiology Kei Ohnuma, Osamu Hosono, Nam H. Dang, and Chikao Morimoto 1. Abstract 2. introduction...