Anticomplementary Activity Of Immunoglobulin

For the measurement of anticomplementary activity (ACA) of immunoglobulin, a defined amount of test material (10 mg of immunoglobulin) is incubated with a defined amount of guinea-pig complement (20 CH50) and the remaining complement is titrated; the anticomplementary activity is expressed as the percentage consumption of complement relative to the complement control considered as 100 per cent.

The haemolytic unit of complement activity (CH50) is the amount of complement that, in the given reaction conditions, will produce the lysis of 2.5 x 108 out of a total of 5 x 108 optimally sensitised red blood cells.

Magnesium and calcium stock solution. Dissolve 1.103 g of calcium chloride R and 5.083 g of magnesium chloride R in water R and dilute to 25 ml with the same solvent. Barbital buffer stock solution. Dissolve 207.5 g of sodium chloride R and 25.48 g of barbital sodium R in 4000 ml of water R and adjust to pH 7.3 using 1 M hydrochloric acid. Add 12.5 ml of magnesium and calcium stock solution and dilute to 5000 ml with water R. Filter through a membrane filter (pore size 0.22 pm). Store at 4 °C in glass containers. Gelatin solution. Dissolve 12.5 g of gelatin R in about 800 ml of water R and heat to boiling in a water-bath. Cool to 20 °C and dilute to 10 litres with water R. Filter through a membrane filter (pore size: 0.22 pm). Store at 4 °C. Use clear solutions only.

Citrate solution. Dissolve 8.0 g of sodium citrate R, 4.2 g of sodium chloride R and 20.5 g of glucose R in 750 ml of water R. Adjust to pH 6.1 using a 100 g/l solution of citric acid R and dilute to 1000 ml with water R. Gelatin barbital buffer solution. Add 4 volumes of gelatin solution to 1 volume of barbital buffer stock solution and mix. Adjust to pH 7.3, if necessary, using 1 M sodium hydroxide or 1 M hydrochloric acid. Maintain at 4 °C. Prepare fresh solutions daily.

Stabilised sheep blood. Collect one volume of sheep blood into one volume of citrate solution and mix. Store at 4 °C for not less than 7 days and not more than 28 days. (Stabilised sheep blood and sheep red blood cells are available from a number of commercial sources.) Haemolysin. Antiserum against sheep red blood cells prepared in rabbits. (Such antisera are available from a number of commercial sources.)

Guinea-pig complement. Prepare a pool of serum from the blood of not fewer than ten guinea-pigs. Separate the serum from the clotted blood by centrifugation at about 4 °C. Store the serum in small amounts below - 70 °C.

Supplements For Diabetics

Supplements For Diabetics

All you need is a proper diet of fresh fruits and vegetables and get plenty of exercise and you'll be fine. Ever heard those words from your doctor? If that's all heshe recommends then you're missing out an important ingredient for health that he's not telling you. Fact is that you can adhere to the strictest diet, watch everything you eat and get the exercise of amarathon runner and still come down with diabetic complications. Diet, exercise and standard drug treatments simply aren't enough to help keep your diabetes under control.

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