Materials

Reagents not specified are of analytical grade.

PBS. Dissolve 8.0 g of sodium chloride R, 0.76 g of disodium hydrogen phosphate R, 0.2 g of potassium chloride R and

0.2 g of potassium dihydrogen phosphate R in water R and dilute to 1000 ml with the same solvent.

PBS-BSA solution. PBS containing 10.0 g/l of bovine albumin R.

Red blood cells. Use D-positive red blood cells obtained from a group O R1R1 donor within 2 weeks of collection. Store if necessary in an appropriate stabiliser at 4 °C. Wash the cells at least twice with PBS-BSA solution and prepare a suspension containing 1 x 104 cells per microlitre but not more than 5 x 104 cells per microlitre in PBS-BSA solution. Use D-negative red blood cells obtained from a group O rr donor and prepared similarly. Secondary antibody. Use a suitable fluorescent dye conjugated anti-IgG antibody-fragment specific for human IgG or parts of it. Store and use according to the manufacturer's instructions.

Microtitres plates. Use flat-bottomed plates without surface treatment for enzyme immunoassays.

Supplements For Diabetics

Supplements For Diabetics

All you need is a proper diet of fresh fruits and vegetables and get plenty of exercise and you'll be fine. Ever heard those words from your doctor? If that's all heshe recommends then you're missing out an important ingredient for health that he's not telling you. Fact is that you can adhere to the strictest diet, watch everything you eat and get the exercise of amarathon runner and still come down with diabetic complications. Diet, exercise and standard drug treatments simply aren't enough to help keep your diabetes under control.

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