Method

Cysteine/cystine is reacted with dithiodiglycolic acid or dithiodipropionic acid to produce a mixed disulphide. The choice of dithiodiglycolic acid or dithiodipropionic acid depends on the required resolution of the amino acid analysis method.

Reducing solution. A 10 g/l solution of dithiodiglycolic acid (or dithiodipropionic acid) in 0.2 M sodium hydroxide.

Procedure. Transfer about 20 pg of the test sample to a hydrolysis tube, and add 5 pl of the reducing solution. Add 10 pl of isopropyl alcohol, and then remove all of the sample liquid by vacuum centrifugation. The sample is then hydrolysed using Method 1. This method has the advantage that other amino acid residues are not derivatised by side reactions, and that the sample does not need to be desalted prior to hydrolysis.

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Supplements For Diabetics

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