System Suitability

Inject the chosen volume of the appropriate system suitability solution.

Average molecular mass of dextran for performance test CRS. Calculate the average molecular mass Mw as indicated under Calibration of the chromatographic system, using either the plotted calibration curve or the values obtained above for b1, b2, b3, b4 and b5. The test is not valid unless Mw is:

- 41 000 to 47 000 (dextran 40 for performance test CRS),

- 67 000 to 75 000 (dextran 60/70 for performance test CRS).

Average molecular mass of the 10 per cent high-fraction dextran. Calculate Mw for the 10 per cent high-fraction dextran eluted through section n using the equation:

in which n is defined by the expressions:

number of sections dividing the chromatograms, height of the chromatographic line above the baseline in section i, molecular mass in section i.

The test is not valid unless Mw of the 10 per cent high fraction dextran is:

- 110 000 to 130 000 (dextran 40 for performance test CRS),

- 190 000 to 230 000 (dextran 60/70 for performance test CRS).

Average molecular mass of the 10 per cent low-fraction dextran. Calculate Mw for the 10 per cent low-fraction dextran eluted in and after section m using the expression:

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Supplements For Diabetics

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