Validation Of The Test

The test is not valid unless the proteins of the molecular mass marker are distributed along 80 per cent of the length of the gel and over the required separation range (e.g. the range covering the product and its dimer or the product and its related impurities) the separation obtained for the relevant protein bands shows a linear relationship between the logarithm of the molecular mass and the Rf. Additional validation requirements with respect to the solution under test may be specified in individual monographs.

QUANTIFICATION OF IMPURITIES Where the impurity limit is specified in the individual monograph, a reference solution corresponding to that level of impurity should be prepared by diluting the test solution. For example, where the limit is 5 per cent, a reference solution would be a 1:20 dilution of the test solution. No impurity (any band other than the main band) in the electropherogram obtained with the test solution may be more intense than the main band obtained with the reference solution.

Under validated conditions impurities may be quantified by normalisation to the main band using an integrating densitometer. In this case, the responses must be validated for linearity.

Supplements For Diabetics

Supplements For Diabetics

All you need is a proper diet of fresh fruits and vegetables and get plenty of exercise and you'll be fine. Ever heard those words from your doctor? If that's all heshe recommends then you're missing out an important ingredient for health that he's not telling you. Fact is that you can adhere to the strictest diet, watch everything you eat and get the exercise of amarathon runner and still come down with diabetic complications. Diet, exercise and standard drug treatments simply aren't enough to help keep your diabetes under control.

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