Concluding Remarks

The liquid-liquid chromatographic method CPC allows for the first time very accurate partition coefficients to be measured in biphasic organic/aqueous systems such as n-octanol/water and n-dodecane/water. The structural information of drug molecules such as hydrogen-bonding capacity can thus be unambiguously revealed using the Alog P

parameter, providing better insight into the mechanisms of drug-biomembrane and drug-receptor interactions.

While the measurable log P range is limited to -3 to 3, it seems appropriate for most drug compounds by suitably modifying experimental conditions, e. g., pH. However, mechanical expertise about the centrifuge is inevitable for the introduction of this novel technique into any laboratory.

Acknowledgements

The authors are most grateful to Dr Y. Ito (NIH, Maryland) for stimulating discussions on the hydrodynamic mechanisms of centrifugal partition chromatography. We thank Dr N. El Tayar and other previous and present collaborators (cited in the references) for their contributions to this project. The technical expertise on the maintenance of the chromatograph of G. Boss is highly appreciated. Figs. 2-5 were prepared by Dr Philippe Vallat. We acknowledge the Swiss National Science Foundation for financial support.

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