Lipophilicity of Peptides and Proteins

RPLC has been used traditionally for the separation and purification of peptides and proteins. It can therefore also be employed to measure the lipophilicity of such macro-molecules [61]. However, conformational effects are important and cannot easily be foreseen. This can be illustrated by considering the retention times of a peptide in which the position of only one amino acid is varied [62], A comparison of retention behavior in reversed-phase chromatography (RPC) and hydrophobic interaction chromatography (HIC) revealed significant differences for proteins [63], The driving force in RPC for proteins is mainly enthalpy-driven, while for HIC this is an entropic process.

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