Effects Of Cfps On Proliferation Of Iec6 Cells

Many sea vegetables have high levels of nutrients and other potentially beneficial components that may be useful for the treatment of various diseases. In particular, the antitumor activities of sea vegetables have been widely studied. For example, an algal lectin from Galaxaura marginata exhibits antibacterial activity (Liao et al., 2003), and palmitic acid isolated from the marine alga Colpomenia sinuosa has antitumor activity (Heiba et al., 1997). Marine algae also contain a large amount of polysaccharides such as alginate, fucoidan, carageenan, and agarose.

Active polysaccharides were extracted from C. fulvescens and subjected to agarose gel electrophoresis (Fig. 13.1) using two-band detection. Cf-PS has sulfate and 3,6-anhydrogalactose content of 28.7% and 18.6%, respectively. In addition, Cf-PS contains monosaccharides xylose (85%) and mannose (15%). Although there are no similar experiments replacing Cf-PS with each individual constituent, Cf-PS was evaluated to check whether it may stimulate the proliferation of normal intestinal cells. Cf-PS induced proliferation of IEC-6 cells, as determined by the MTS assay (Fig. 13.2A). After exposure to 0-1000 mg/ml Cf-PS for 24 h, relative cell numbers increased in a concentration-dependent manner because exposure to 500 mg/ml of Cf-PS for 24 h was sufficient to stimulate

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1 2

FIGURE 13.1 Agarose gel electrophoresis of the Cf-PS. Lane 1, 30 mg Cf-PS; lane 2, 60 mg Cf-PS.

FIGURE 13.1 Agarose gel electrophoresis of the Cf-PS. Lane 1, 30 mg Cf-PS; lane 2, 60 mg Cf-PS.

growth. Cell morphology results showed that cells treated with Cf-PS appeared to increase in number compared to untreated cells (Fig. 13.2B). Further, expression of PCNA increased in treated cells (Fig. 13.2C), confirming that Cf-PS induced growth of IEC-6 cells.

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