O

FIGURE 15.6 Scheme for the glucose transmission test of immature LJE in Caco-2 cells. Caco-2 cells were precultured for 2 weeks to cover the membrane filter as an experimental model for intestinal epithelia in vitro. Completion of the cell monolayer was detected by TEER measurement (Millicell-ERS). The transport of glucose from the upper cavity (apical site) to the lower cavity (basal site) through glucose transporters was evaluated by measuring both glucose concentrations for constant periods.

8,000 T

o 2,000

Control Phloridzin LJ LJ

100 |iM 100 mg/ml 200 mg/ml

FIGURE 15.7 Effect of immature LJE on D-[6-3H] glucose intake in Caco-2 cells. To measure the amount of glucose intake in Caco-2 cells, tritium-labeled glucose and immature LJE were added to the culture medium. Phloridzin inhibits sodium-dependent glucose transporters. Data are expressed as the average ± SE (n — 4). p < 0.005 versus control.

FIGURE 15.7 Effect of immature LJE on D-[6-3H] glucose intake in Caco-2 cells. To measure the amount of glucose intake in Caco-2 cells, tritium-labeled glucose and immature LJE were added to the culture medium. Phloridzin inhibits sodium-dependent glucose transporters. Data are expressed as the average ± SE (n — 4). p < 0.005 versus control.

Time (min)

FIGURE 15.8 Effects of immature LJE on glucose transmission in Caco-2 cells. To measure the amount of glucose throughout the monolayer of Caco-2 cells, the amounts of glucose in the lower layer of the transwell were measured with or without immature LJE. Data are expressed as the average ± SE (n = 4). < 0.05, **p < 0.01, ***p < 0.005 versus control.

Time (min)

FIGURE 15.8 Effects of immature LJE on glucose transmission in Caco-2 cells. To measure the amount of glucose throughout the monolayer of Caco-2 cells, the amounts of glucose in the lower layer of the transwell were measured with or without immature LJE. Data are expressed as the average ± SE (n = 4). < 0.05, **p < 0.01, ***p < 0.005 versus control.

On the other hand, LJ suppressed glucose transmission from the upper layer to the lower layer in a concentrate-dependent manner, as shown in Fig. 15.8.

Caco-2 cells express sodium-dependent transporter 1 (SGLT1) in microvillus membrane and glucose transporter 2 (GLUT2) in the whole cell. This transporter operates via the glucose concentration in the small intestine. Mainly, SGLT1 or GLUT2 transports glucose from the gut lumen to cells and GLUT2 transports glucose from the apical side to the basolateral side. These results suggest that immature LJE did not affect glucose intake in Caco-2 cells but significantly suppressed the transmission of glucose in monolayer membranes by inhibiting GLUT2.

The results of the oral carbohydrate tolerance test and the long-term administration test suggest that immature LJE suppresses the postprandial blood glucose level. These results suggest that immature LJE can be used as a food for the prevention of diabetes. The active component in LJE remains to be elucidated.

How To Bolster Your Immune System

How To Bolster Your Immune System

All Natural Immune Boosters Proven To Fight Infection, Disease And More. Discover A Natural, Safe Effective Way To Boost Your Immune System Using Ingredients From Your Kitchen Cupboard. The only common sense, no holds barred guide to hit the market today no gimmicks, no pills, just old fashioned common sense remedies to cure colds, influenza, viral infections and more.

Get My Free Audio Book


Post a comment