Abbreviations

AP, allophycocyanin; DBV, 15,16 dihy-drobiliverdin; DTT, dithiothreitol; EDTA, ethylenediamine tetraacetate; HPLC, high-performance liquid chromatography; MBV, mesobiliverdin; PC, phycocyanin; PCB, phycocyanobilin; PE, phycoerythrin; PEB, phycoerythrobilin; PEC, phycoery-throcyanin; PUB, phycourobilin; PXB, phycobiliviolin; P^B, phytochromobilin; TFA, trifluoroacetic acid.

Figure 7. HPLC elution profile from a Ci8-reverse phase column of a tryptic digest of a preparation of Synechococcus sp. PCC 7002 apophycocyanin after reaction with free PCB, in the absence of enzymes. The major products are MBV at the a-84 (a-1MBV) and P-82 (P-1MBV) sites with some PCB forming at the P-82 site (P-1PCB; the amount of this product is variable). The elution of bilin-linked peptides was monitored at 660 nm. This figure was modified with permission from Reference 2.

Figure 7. HPLC elution profile from a Ci8-reverse phase column of a tryptic digest of a preparation of Synechococcus sp. PCC 7002 apophycocyanin after reaction with free PCB, in the absence of enzymes. The major products are MBV at the a-84 (a-1MBV) and P-82 (P-1MBV) sites with some PCB forming at the P-82 site (P-1PCB; the amount of this product is variable). The elution of bilin-linked peptides was monitored at 660 nm. This figure was modified with permission from Reference 2.

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