DTT, dithiothreitol; EDTA, ethylene diamine tetra acetic acid; HO-1, human heme oxygenase isozyme 1; HRP, horseradish peroxidase; HSAP, hemoprotein spec tral analysis program; IEF, isoelectric focusing; H2O2, hydrogen peroxide; LDS, lithium dodecyl sulfate; MCD, magnetic circular dichroism; NMR, nuclear magnetic resonance; PAGE, polyacrylamide gel electrophoresis; PCA, principal component analysis; PMSF, phenylmethylsulfonyl fluoride; PVDF, polyvinylene difluoride membrane; SDS, sodium dodecyl sulfate; SVD; singular value decomposition analysis; TCEP, tris(2-carboxyethyl) phosphine; TMBZ, 3,3 ' ,5,5 ' -tetramethylbenzidine.

Figure 6. Electrophilic oxidation of the porphyrin macrocycle by the ferric peroxide complexes.
Figure 7. Schematic representation of the unpaired spin distribution about the heme periphery in HO-1 and myoglobin. The size of the circle represents the population of the spin distribution. Adapted from Reference 33.
0 0

Post a comment