Future Developments

Further development in MS can be expected with the introduction of user-

Figure 10. Separation of porphyrins in the urine of a patient with CEP by CE. Running buffer, 100 mM SDS, 20 mM CAPS (pH 11.0); voltage, 25 kV; temperature, 450°C; detection, absorbance 404 nm. Peaks: 4, 5, 6, 7, and 8 refer, respectively, to tetra(copro)-, penta, hexa-, hepta-, and octa(uro)car-boxylic acid porphyrin.

Figure 10. Separation of porphyrins in the urine of a patient with CEP by CE. Running buffer, 100 mM SDS, 20 mM CAPS (pH 11.0); voltage, 25 kV; temperature, 450°C; detection, absorbance 404 nm. Peaks: 4, 5, 6, 7, and 8 refer, respectively, to tetra(copro)-, penta, hexa-, hepta-, and octa(uro)car-boxylic acid porphyrin.

friendly instruments of very high sensitivity and resolution. This will allow the porphyrins to be analyzed with even greater sensitivity and specificity, especially in HPLC-MS/MS analysis. The use of microcolumn HPLC-nanospray MS will further enhance the sensitivity of detection and should be explored for possible application in the analysis of the biosynthetic intermediates from ALA to heme.

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