Contents

2.1 Introduction

2.2 In Vitro Neurochemical Techniques

2.2.1 Superfusion

2.2.2 Slices or Synaptosomes?

2.2.3 Subtypes of Presynaptic nAChR

2.3 In Vitro Electrophysiological Approaches

2.4 In Vivo Neurochemical Methods

2.5 nAChR Localization

2.5.1 Receptor Ligand Binding

2.5.2 nAChR Subunit Expression

2.5.3 Immunocytochemistry

2.5.3.1 General Principles

2.5.3.2 Immunocytochemistry at the Light/ Confocal Microscope Level

2.5.3.3 Electron Microscopy

2.6 Conclusions References

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