Contents

1.1 Introduction

1.2 Maintenance of Cell Lines Naturally Expressing nAChR

1.3 Creation and Maintenance of Cell Lines Stably and Heterologously Expressing nAChR of Defined Subunit Composition

1.3.1 Vector Selection, Transfection, and Subcloning

1.3.2 Host Cell Types Used and nAChR Subtypes Expressed

1.4 Isotopic Ion Flux Assays for Rapid Characterization of Functional nAChR Ensembles

1.4.1 Efflux and Influx Assays

1.4.2 Techniques for Cell Manipulation

1.5 Radioligand Binding Assays

1.5.1 Intact Cells — Suspension and In Situ

1.5.2 Membranes and Detergent-Solubilized Preparations References

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