Figure 7.55 Intraplate errors in PAMPA measurement in 2% DOPC model.

7.7.15 UV Spectral Data

The use of direct UV spectrophotometry to measure sample concentrations in pharmaceutical research is uncommon, presumably because of the prevalence and attractiveness of HPLC and LC/MS methods. Consequently, most researchers are unfamiliar with how useful direct UV can be. The UV method is much faster than the other methods, and this is very important in high-throughput screening.

If samples are highly impure or decompose readily, the UV method is inappropriate to use. LC/MS has been demonstrated to be a suitable detection system

TABLE 7.21 Approximate Intraplate Errors in PAMPA Measurement"

Pe(10~6 cm/s)




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