Cloning Of The Opioid Receptor Family

The cloning of the delta receptor set in motion a competitive race to identify other members of the opioid receptor family. Homologous orphan clones were quickly assessed for opioid receptor binding properties, which resulted in the identification of the kappa receptor and reassignment of an orphan clone as the delta opioid receptor [31]. PCR, genomic, and cDNA screens revealed the mu opioid receptor and an extremely abundant orphan member, named opioid receptor-like (ORL-1) receptor (reviewed by Massotte and Kieffer [30]).

DELTA ---------------------ME PAPS-AGAELQ-PPL FANAS DAY-----PSAFPSASANAS GPPGARS ASS

HU HDS S AAPTNASNCTD ALAYSSCSP APS -PGSWVNLS HL DGNLS DPCG----PNRTDLGGRDSLCPP — TGSPS

KASP A --------KJ5SMQI PRO----BPGPTCA» S ACL-PPNSS AwfP GWAJS P--PSNOSASSSOAQLES---AHIS

ORL-1 -----------------------HEPL-FPAPFW-EVIYGSHLQGNLSLLSPNHSLLPPHLLLNAS-HSAFLP

DELTA JJU^IAITM.ÏaAVtAVÛl.LÛNVI.VH^alVHÏTKWKMilIÏIf'NLALAiJAIJi'rHTI.Pl'gSAISÏIJUâTWt'FQK MU M I TAI TIMA1>Y51VC] WGLFGNFDVMYVIV RYTKMKTATN 1YIF N LiAliADAliATS TIiP FQSVNYL MGTWF FGT

ORL-1 LGLKVTIVGLÏLAVCVGGLLGNCLVHYVII.RHTKMKTATHIÏirNLALAIJTLVLLTUFQGTDILLGFWPP5N

DE LTA LLC KAVLS Iföf YMMFTSIFTHHSTORYI AVCHFVKALDFRTPMUUnllNIC IWV1ASGVGVP11JVHAVTW

HU ILCKIVISID Y YNHFT3IFTLCTMSV DRY!AVCHP VK AL D FRTPRNAK11NVCNWILS SAIGLPVMFHATTKY

KAPPA VLC KIVI51DY Y HHF'TS T FTTjTHHS VDRYI AVCHPVKAI.DFKTPIiK A* IT NTC T WLLE SSVG T 3 ATVLGGTKV

ORL-1 ALCKTVtAIDYYNr^FTSTFT LTAMSVDRYVAICHP I RALD VRTËSK ASAVHVAI KALA 5WGVPVAIMSSAQV

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