[15 Redox Control of Integrin Adhesion Receptors

By Jeffrey W. Smith, Boxu Yan, France Landry, and Christian R. Lombardo

Reagents

The following is a list of reagents that have been used in our studies of the integrin redox site. The manufacturer of each reagent is provided.

Triton X-100 (Sigma, St. Louis, MO) Phenylmethylsulfonyl fluoride (PMSF; Sigma) Leupeptin (Sigma)

Methyl-a-d-mannopyranoside (Sigma) Guanidine-HCl (Sigma) Trichloracetic acid (Sigma) Dimethyl sulfoxide (Sigma) Glutathione (reduced form; Sigma) l-Cysteine (Sigma)

Avidin-horseradish peroxidase (HRP) (Sigma)

Concanavalin A (ConA)-Sepharose (Amersham Pharmacia Biotech,

Piscataway, NJ) CNBr-Sepharose (Amersham Pharmacia Biotech) Aquacide (CalBiochem-Novabiochem, La Jolla, CA) [14C]Iodoacetamide (New England Nuclear, Boston, MA) Dimethylformamide (Aldrich, Milwaukee, WI)

full enzymatic pathway, in concert with detection of these activities in lysates of Percoll-purified spirochetes, will be required to conclusively delineate the components of the treponemal oxidative defense pathway(s). Moreover, comparative functional analysis of these pathways in vivo and in vitro may reveal a relationship between treponemal oxidative stress responses and syphilis pathogenesis. These lines of investigation hold the potential to yield knowledge essential to the long-sought continuous cultivation of T. pallidum in vitro.

Acknowledgments

This research was supported in part by NRS a postdoctoral fellowship award ai-10573 to K.R.O.H., U.S. Public Health Service Grant ai-26756 to J.D.R., and by support from the Mayo Foundation to F.R.

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